Skip to main content
. 2005 Mar;4(3):588–603. doi: 10.1128/EC.4.3.588-603.2005

FIG. 8.

FIG. 8.

FIG. 8.

FIG. 8.

Cdc24 is recruited to the tips of germ tubes in response to FCS. (A) Cdc24GFP is functional. The indicated strains were grown on medium which permits (−Met/Cys) or represses (+Met/Cys) MET3-driven CDC24 expression for 2 days at 30°C. (B) Immunoblot analysis of cdc24Δ/PMETCDC24 mutant cells with or without integrated Cdc24GFP. Extracts from cells grown at 30°C were analyzed with anti-GFP polyclonal antibodies. (C) Cdc24GFP localization over 120 min in cells in the presence of FCS. cdc24Δ/PMETCDC24 cells overexpressing Cdc24GFP were grown in YEPD-Uri medium with Met and Cys at 30°C (Budding) and for the indicated times in FCS at 37°C. DIC and fluorescence images are shown. Panels show individual cells after 60 and 120 min in FCS at 37°C. (D) Cdc24GFP localization in the presence or absence of FCS. cdc24Δ/PMETCDC24 cells overexpressing Cdc24GFP grown in SC medium with Met and Cys for 60 min in the presence or absence of FCS were examined by confocal microscopy. DIC images are average intensity projections, and fluorescence images are maximum intensity projections of 15 to 17 0.2-μm z sections.