FIG. 4.

Regulation of TRP2 transcriptional activity by PKA involved the M box (−129 to −135), the CRE-like motif (−239 to −232), and the E box (−346 to −340). B16 cells were transfected with 0.3 μg of pTRP2, mMBOXpTRP2, and Δ1pTRP2 (A) or Δ2pTRP2, Δ3pTRP2, Δ4pTRP2, mCREpTRP2, and mEBOXpTRP2 (B), 0.2 μg of pCDNA3, empty or encoding PKA, and 0.05 μg of pCMVβGAL. After 48 h, luciferase activity was assayed as described in the text and normalized to β-galactosidase activity. ▿, TATA-box position. Results are expressed as fold stimulation of the basal luciferase activity from unstimulated cells. Data are means ± standard errors of five experiments performed in triplicate.