FIG. 7.
cAMP increases microphthalmia expression in B16 mouse melanoma cells. B16 melanoma cells and NIH 3T3 cells nontransfected or transfected with microphthalmia were labeled with [35S]methionine-cysteine mix for 30 h. (A) NIH 3T3 cells were solubilized and immunoprecipitated with antimicrophthalmia antibody. (B) B16 melanoma cells were exposed to 20 μM forskolin for the indicated time, and then solubilized proteins were immunoprecipitated with antimicrophthalmia antibody. The immune complexes were analyzed by gel electrophoresis and autoradiography. Molecular masses, indicated on the left, are expressed in kilodaltons.