TABLE 1.
Yeast strainsa
| Strain | Genotype | Source or reference |
|---|---|---|
| Y3690 | MATaHIS4 leu2-kho::LYS2ura3-1trp1::hisGlys2MATα his4 leu2::hisGho::LYS2 ura3-1 trp1::hisG lys2 | This study |
| Y592 | Y3690 but homozygous sps1::TRP1 | This study |
| Y3710 | Y592 + 2μm GFP-SPO14 LEU2 (pME1096) | This study |
| Y4998 | Y3690 + 2μm GFP-SPO14 LEU2 (pME1096) | This study |
| Y4145 | Y3690 + CEN4 SPR28-GFP URA3 (pSB19) | This study |
| Y4146 | Y592 + CEN4 SPR28-GFP URA3 (pSB19) | This study |
| YKS53-1 | MATaLEU2ho::hisGura3lys2DON1-eGFP-kanMXMATα leu2::hisG ho::hisG ura3 lys2 DON1-eGFP-kanMX | 11 |
| Y3947 | YKS53-1 but homozygous sps1::URA3 | This study |
| Y3059 | YKS53-1 but homozygous spo14::URA3 | This study |
| AN120 | MATaleu2ARG4RME1hoΔ::LYS2ura3trp1::hisGlys2 MATα leu2 arg4-NspI rme1Δ::LEU2 hoΔ::LYS2 ura3 trp1::hisG lys2 | 17 |
| Y4535 | AN120 but homozygous CHS3-GFP | This study |
| Y4537 | AN120 but homozygous GSC2-GFP | This study |
| Y4584 | Y4537 but homozygous sps1::TRP1 | This study |
| Y4620 | Y4535 but homozygous sps1::TRP1 | This study |
| Y4745 | AN120 but homozygous DTR1-GFP | This study |
| Y4741 | Y4745 but homozygous sps1::TRP1 | This study |
| Y4748 | AN120 but homozygous dtr1::kanMX | This study |
| Y4749 | Y4748 but homozygous sps1::TRP1 | This study |
| Y4743 | AN120 but homozygous SHC1-GFP | This study |
| Y4746 | Y4743 but homozygous sps1::TRP1 | This study |
| Y4716 | Y4535 but homozygous shc1::kanMX | This study |
| Y5050 | AN120 but homozygous GFP-SPS1 | This study |
| Y5088 | AN120 but heterozygous GFP-SPS1 and CHS3-dsRed | This study |
a
All strains are of SK1 background.