FIG. 6.

Rox1 is required for Mot3 binding to the ANB1 gene and, to a lesser extent, to the HEM13 gene. ChIP assays with monoclonal antibody against the HA epitope were performed with RZ53-6Δmot3 transformed with YCp(23)MOT3-HA (WT) or YCp(23)MOT3 (untagged, UT) and with RZ53-6Δrox1Δmot3 cells transformed with YCp(23)MOT3-HA (rox1Δ). Cells were grown aerobically to mid-exponential phase. DNA samples were prepared before (Input) and after (ChIP) immunoprecipitation. The radioactivity in the bands was quantitated and normalized as described in the legend to Fig. 5.