TABLE 6.
Repression by the Rox1-Ssn6 fusion
| Genotypea | Result for:
|
|
|---|---|---|
| ROX1b | ROX1-SSN6c | |
| SSN6 | ||
| rox1Δ | 3.3 ± 0.9 | 0.9 ± 0.1 |
| rox1Δ mot3Δ | 14 ± 1.4 | 4.3 ± 0.3 |
| rox1Δ tup1Δ | 67 ± 10 | 143 ± 28 |
| ssn6Δ | ||
| rox1Δ ssn6Δ | 103 ± 5 | 4.7 ± 0.2 |
| rox1Δ mot3Δ ssn6Δ | 128 ± 12 | 5.9 ± 0.9 |
a
The enzyme assays were carried out in extracts from cells containing an integrated ANB1-lacZ fusion in derivatives of MZ22-4 for SSN6 or KZ211 for ssn6Δ. The numbers shown represent Miller units ± standard deviations.
b
ROX1 denotes transformants expressing a wild-type ROX1 plasmid.
c
ROX1-SSN6 denotes transformants expressing the ROX1-SSN6 fusion.