Fig 5.

Proviral sequences which can be generated with the G1-form HIV-1 transcripts as template were dominantly found. (A) A schematic represents the GGG tract located at the U3/R junction in 3’LTR of HIV-1 clone DNA (pNL4-3EGFPΔenvΔnef). Nucleotide sequences of the 3′ LTR TTT mutant of NL4-3EGFPΔenvΔnef are shown. The mutated nucleotides are highlighted by red bold letters. (B) Sequencing analyses of proviral sequences after infection with the VSV-G-pseudotyped NL4-3EGFPΔenvΔnef 3’LTR TTT mutant are shown (n = 46 clones). TF input: a plasmid for transfection to express HIV-1 transcripts (pNL4-3EGFPΔenvΔnef-based mutant plasmid).