FIG. 3.

Immunoreactivity of glycosylation-deficient C. albicans derivatives with anticarbohydrate antibodies. Cell wall proteins from stationary phase cultures of C. albicans glycosylation mutants were extracted by digestion with Zymolyase 100T (a β-1,3-glucanase; ICN Biochemicals) and separated by on 12% polyacrylamide gels for transfer to nitrocellulose. Western blots were probed either with MAb B6.1, which recognizes a β-1,2-mannotriose, or MAb B6, which recognizes an incompletely characterized epitope in the acid-stable mannan fraction. Lanes were loaded with equal amounts of protein, which resulted in a consistent pattern across the blot as visualized by staining with Ponceau S. The sample on the right (LGH1095*) represents total cell lysate generated by glass bead breakage of a cell pellet (49).