FIG. 5.

Acid-stable mannan analysis of C. albicans derivatives by purification and FACE. Bulk mannan was prepared as before from wild-type (LGH1095 and A9) and mnn4Δ/mnn4Δ cells grown at either 25°C (hydrophobic cells) or 37°C (hydrophilic cells). The α-1,6-mannose backbone of purified acid-stable mannan was cleaved by acetolysis as described in the text, and equal moles of cleaved reducing ends were labeled with ANTS and electrophoretically separated as described in the legend of Fig. 4. The left-hand lane is a labeled ladder of hydrolyzed dextran (α-1,6-glucose) as an approximate sizing standard, and the right-hand lane is a acid-labile ladder from phobic cells prepared as described in the legend of Fig. 4. wt, wild type; Dex, dextran; AL, acid-labile ladder; Phil, hydrophilic; Phob, hydrophobic; KO, knockout.