TABLE 2.
Oligonucleotides
| Name | Sequence | Reference |
|---|---|---|
| MTLa F | TTGAAGCGTGAGAGGCAGGAG | 23 |
| MTLa R | GTTTGGGTTCCTTCTTTCTCATTC | 23 |
| MTLαF | TTCGAGTACATTCTGGTCGCG | 23 |
| MTLαR | TGTAAACATCCTCAATTGTACCCGA | 23 |
| 5 MRS RB2√ | CTATTACAAATTACACAAGCGCCTGAG | This work |
| 5 MRS HOK√ | GACAGAATCATCCCACCCTGACT | This work |
| 3RB2 √ F | CACTACGGATTCTAATAACCCAGCAC | This work |
| 3RB2 √ R | GACATACCCAAGACATTTAGGACGC | This work |
| URA3 DETECT R | TCACCAGTAGCACAACGAT | This work |
| URA3 DETECT F | TCCAACTCCAGGTGTCAT | This work |
| 5RB2 MRSΔ | TTTTTAATTAACCTGTACATGATTTGGAACTGGGGCAACAGTTGTCCCTTCTCCCTGTCTAGAAGGACCACCTTTGATTG | This work |
| 5HOK MRSΔ | ACCACAAATTATTAGTGGTTGTTTGCTTCATCACAACTGCATGCTTAATCCTCGCTAATGCTGGTAAAGATTCGAACGAC | This work |
| 3′ Chr3RB2Δ | GGCGAATTCAAATGGATATGTTATTATTCCTTCAACTGCTCCTGCAGTTCCACCAGCTCTAGAAGGACCACCTTTGATTG | This work |
| 5′ Chr3RB2Δ | CAAATCGGAAACTTCACAGGGTTATGGTTGTTGCAACCTAACGTATTTTTTGCCTAATGCTGGTAAAGATTCGAACGAC | This work |