Figure 3.

Cas-SF01 displays high editing efficiency and a broadened PAM range

(A) Cas-SF01 exhibits higher cleavage activity than wild-type Cas12i3, Cas12i^HiFi, and hfCas12Max in the EGxxFP-reporter system. T1–T9 loci, target sites in the inserted fragment of the EGxxFP cassette. ∗∗∗p < 0.001; ∗∗p < 0.01; ns, not significant (Student’s t test).

(B) Comparison of indel activity of Cas-SF01 with SpCas9, AsCas12a Ultra, and hfCas12Max at multiple target sites in the PCSK9, CCR5, and TTR loci. Significant difference was determined by Student’s t test.

(C) Cas-SF01 shows improved recognition of targets with an NATN PAM compared with wild-type Cas12i3.

(D) Heatmap showing the PAM recognition profile of Cas-SF01.