Figure 3.

CSN5 protects platinum-resistant EOC cells from platinum injury by regulating ER stress signaling. Proteomic analysis was applied to identify the O-GlcNAcylated proteins in platinum-resistant EOC cells with or without CSN5 knockdown, and functional characterization of the identified O‑GlcNAc proteins was analyzed. (A, B) Significantly enriched biochemical and signaling pathways of the identified O-GlcNAcylated proteins were revealed by integrated GO and KEGG pathways analysis (p < 0.05). (C, F) qRT-PCR analysis of the representative gene expression contained in the ER stress signaling pathway in DDP cells with or without CSN5 inhibition. (D, G) IB analysis of the representative ER stress signaling protein levels. (E, H) Densitometric analysis of protein expression contained in the ER stress signaling pathway. Experiments were performed in triplicate.