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The expression of pldB is developmentally regulated. (A) A Northern blot of total cellular RNA isolated from Ax2 cells at 4-h intervals after starvation on filter pads was probed with a radiolabeled fragment of the pldB gene. (B) A Western blot of lysates from pldB− cells and Ax2 cells taken at 4-h intervals after starvation on filter pads was probed with affinity-purified anti-PldB peptide antibodies.
