TABLE 1.
2D-DIAS analysis of basic motile behavior when perfused with BSS in the absence of chemoattractanta
| Parameter | Value for strain
|
Pb | |
|---|---|---|---|
| JH10 | acaA− | ||
| Motility parameters | |||
| No. of cells | 20 | 33 | |
| Instantaneous velocity (μm/min) | 7.8 ± 2.8 | 8.3 ± 3.2 | NS |
| Directional persistence | 0.36 ± 0.20 | 0.37 ± 0.19 | NS |
| Directional change (°) | 48 ± 16 | 41 ± 12 | NS |
| Positive flow (%/5 s) | 9.4 ± 2.7 | 9.8 ± 2.4 | NS |
| Negative flow (%/5 s) | 9.0 ± 2.6 | 9.2 ± 2.1 | NS |
| Shape parameters | |||
| No. of cells | 20 | 33 | |
| Maximum width (μm) | 9.8 ± 2.0 | 10.2 ± 2.0 | NS |
| Maximum length (μm) | 22.5 ± 4.5 | 18.2 ± 3.5 | 1 × 10−3 |
| Roundness (%) | 47 ± 16 | 64 ± 8 | 1.4 × 10−4 |
| Mean convexity (°) | 653 ± 130 | 531 ± 91 | 8.9 × 10−4 |
| Mean concavity (°) | 294 ± 131 | 171 ± 91 | 9.2 × 10−4 |
| Radial deviation (%) | 37 ± 8 | 28 ± 4 | 1.9 × 10−4 |
a
Parameters (means ± standard deviations) are calculated according to the method of Soll (30).
b
P values of ≥0.05 were considered nonsignificant (NS).