TABLE 2.
Frequency of lateral pseudopod formation in buffer, in spatial gradients of cAMP, and in the front of simulated temporal waves of cAMP
| Cell type | Frequency of lateral pseudopod formation per 10 mina
|
||
|---|---|---|---|
| In buffer | In spatial gradient of cAMP | In front of temporal wave (10−8 to 10−6 M) | |
| JH10 | 5.1 ± 2.7 (13) | 0.6 ± 1.2 (11) | 1.1 ± 2.1 (19) |
| acaA− | 4.6 ± 2.4 (27) | 4.2 ± 1.7 (26) | 3.1 ± 3.0 (29) |
| acaA− (r) | 4.7 ± 1.2 (23) | 4.9 ± 2.7 (17) | 2.8 ± 3.7 (20) |
The method for assessing pseudopod frequency can be found in Wessels et al. (47). Frequencies are provided as means ± standard deviations. Individual cells (total numbers of cells examined are given in parentheses) were analyzed for 10-min periods. P values were computed by a Student's t test; a P value of >5 × 10−2 was considered nonsignificant. P values for the comparison of JH10 cells with acaA− and acaA− (r) cells were nonsignificant in buffer; in spatial gradient, the P values were 6 × 10−8 and 2 × 10−6, respectively, and in front of the temporal wave, they were 4 × 10−6 and 2 × 10−3, respectively. In buffer versus spatial gradient, the P values were 4 × 10−5 for JH10 cells and nonsignificant for acaA− and acaA− (r) cells. In buffer versus the front of the temporal wave, the P values were 1 × 10−4 for JH10 cells, 1 × 10−2 for acaA− cells, and 6 × 10−2 for acaA− (r) cells.