TABLE 4.
Cell type | No. of cells | Concn of cAMP (M)
|
% of cells exhibiting velocity surge in front of:
|
|||
---|---|---|---|---|---|---|
Peak | Trough | Wave 2 | Wave 3 | Wave 4 | ||
Control JH10 | 19 | 10−6 | 10−8 | 68 | 63 | 79 |
acaA− | 38 | 10−6 | 10−8 | 29 | 42 | 68 |
acaA− (r) | 28 | 10−6 | 10−8 | 49 | 39 | 64 |
Control JH10 | 11 | 10−5 | 10−7 | 73 | 55 | 82 |
acaA− | 16 | 10−5 | 10−7 | 75 | 63 | 75 |
Control JH10a | 20 | 0 | 0 | 5 | 5 | 0 |
In this control, JH10 cells were analyzed while continually being perfused with buffer lacking chemoattractant and analyzed during time periods comparable to the fronts of temporal waves.