Fig 2.

AMCase in acidic conditions promotes Ascaris larval hatching: (A) Ascaris eggs were treated with recombinant AMCase under acidic and neutral pH in vitro. Larval hatch rate was quantified post treatment. (B-C) BALB/c mice were challenged by oral gavage with 2,500 eggs of Ascaris and stomach tissue was harvested 1 hour or 1 day post infection to extract total RNA. Chia1 (B) and Atp4a (C) expression level were quantified by qPCR. (D) BALB/c mice were pretreated with AMCase inhibitor, omeprazole, 10% NaHCO₃ or vehicle control before challenged by oral gavage with 2,500 eggs of Ascaris. (E-F) Larva isolated from the liver 4 days post infection under different conditions was quantified. (n≥4, mean±S.E.M, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 using two-way ANOVA followed by Tukey’s test for multiple comparison (A), two-tailed Student’s t-test (B, C, E) or one-way ANOVA followed by Tukey’s test for multiple comparison (F). Data are shown as representative of two independent experiments. Illustration generated by Biorender.com).