Figure 6.

GFP-Nit1 aggregation and callose induction by bromoxynil. (A) 8 day old GFP-Nit1 plants (N1P2E) seedlings were imbibed in 500 μM Bromoxynil (+Bx) or mock solutions (-Bx) for 1 h then imaged by confocal microscopy, note the exstensive accumulation of the fusion protein in aggregates after herbicide treatment. (B) Callose accumulation following bromoxynil treatment. Wild type seedlings were treated with 500 μm bromoxynil (+Bx) or a mock-solution (-Bx). Roots were examined at regular intervals under UV illumination using conventional epifluorescence and a DAPI filter set; the 15 min time point is shown. Callose deposition was visualized using the callose specific stain sirofluor, added at 100 ug/ml at the beginning of the experiment. Sirofluor fluorescence was first visible approximately 8 min after addition of bromoxynil. Images were acquired on a digital camera using identical exposure settings.