Figure 8.

ER Fusiform body alterations during wound and chloroxynil induced cell death. (A) Hypocotyl cell of an unwounded plant showing the normal morphology of the fusiform bodies. (B) Wound proximal hypocotyl cell. Note the spherical bodies in this cell, distinct from the normally cigar-shaped structures illuminated by lumenal ER markers. (C) Chloroxynil-induced transitions. Epidermal hypocotyl cells were treated with 500 μM chloroxynil. Inset numbers indicate time (in minutes) after herbicide addition. An individual fusiform body throughout the experiment is marked by the red arrow. By 4 min after herbicide application, streaming ceases, evident by the lack of movement of fusiform bodies and the tubular ER network, which are usually highly dynamic. Shortly thereafter, fusiform bodies transform into spherical structures. Scale bar = 10 μm.