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. 2005 Mar 21;2:20. doi: 10.1186/1742-4690-2-20

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Copyright © 2005 Liang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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CAT assays with HIV-LTR-CAT and its activator Tat, and HTLV-LTR-CAT and its positive activator Tax. Lymphocyte (CEM, 12D7) cells were grown to mid log phase and were processed for electroporation according to a procedure published previously [52]. The cells were washed with phosphate-buffered saline and resuspended in RPMI 1640. They were next transfected with reporter constructs (HIV-LTR-CAT or HTLV-LTR-CAT; 3 ug of each), their respective activators (Tat or Tax; 4 ug each) or with various siRNAs (10 ug each). Lanes 1–3 serve as positive controls for basal, activated transcription and effect of cdk2 siRNA on inhibition of HIV-1 LTR. Lanes 4–14 are basal, activated transcription and effect of various siRNAs on HTLV- LTR-CAT. Only cdk9 siRNA showed an appreciable amount of suppression on Tax activated HTLV-LTR (lane 8). CAT % conversations are listed below the diagram.