图 4.

转染siRNA-C/EBPβ对各组细胞C/EBPβ表达的影响

C/EBPβ expression in each cell line after transfection with siRNA-C/EBPβ. A: Relative C/EBPβ mRNA level in cells transfected with siRNA-C/EBPβ was reduced, especially in the NPHP1^KDHK2 cell line (n=6). B: Western blotting of C/EBPβ protein in NPHP1WT and NPHP1^KD HK2 cells. The expression of C/EBPβ subunits in the transfected siRNA-C/EBPβ cells is significantly reduced. C-E: Relative LAP^*/LAP/LIP protein levels adjusted to β-tubulin. The protein expressions of LAP^* and LIP subunits are reduced in cells transfected with siRNA-C/EBPβ, especially in the NPHP1^KD HK2 cell line. ^*P < 0.05; ^***P < 0.001; ^****P < 0.0001.