表 3.
qPCR引物序列
Sequences of qPCR primers
| Target gene | qPCR primer (5'→3') | Amplification products | GC (%) | Temperatures of anneal |
| GAPDH | GGAGCGAGATCCCTCCAAAAT (Upstream) | 197 bp | 52.38 | 59.86 ℃ |
| GGCTGTTGTCATACTTCTCATGG (Downstream) | 47.83 | 59.38 ℃ | ||
| NPHP1 | GCTACAGAAGGCACTATTAGGT (Upstream) | 177 bp | 45.45 | 57.33 ℃ |
| TGTGGCTCTGACTGTATGAATG (Downstream) | 45.45 | 58.13 ℃ | ||
| TNF-α | GCTGCACTTTGGAGTGATCG (Upstream) | 108 bp | 55.55 | 59.55 ℃ |
| TCACTCGGGGTTCGAGAAGA (Downstream) | 55.55 | 60.25 ℃ | ||
| C/EBPβ | GGAGCCCGTCGGTAATTT (Upstream) | 76 bp | 55.56 | 57.05 ℃ |
| TCTGCATGTGCGGTTGG (Downstream) | 58.82 | 57.87 ℃ | ||
| CCL2 | AGCCAGATGCAATCAATGCC (Upstream) | 119 bp | 50.00 | 59.25 ℃ |
| AGCTTCTTTGGGACACTTGCT (Downstream) | 47.62 | 60.13 ℃ | ||
| IL-1β | ACAGTGGCAATGAGGATG (Upstream) | 129 bp | 50.00 | 54.54 ℃ |
| TGTAGTGGTGGTCGGAGA (Downstream) | 55.56 | 56.74 ℃ | ||
| IL-6 | GTACATCCTCGACGGCATC (Upstream) | 98 bp | 57.89 | 57.82 ℃ |
| TCAGGTTGTTTTCTGCCAGT (Downstream) | 45.00 | 57.56 ℃ | ||
| CCL20 | GCGAATCAGAAGCAGCAAGC (Upstream) | 116b p | 55.00 | 60.52 ℃ |
| GATGTCACAGCCTTCATTGGC (Downstream) | 52.38 | 59.87 ℃ | ||
| CXCL5 | CAGACCACGCAAGGAGTTCA (Upstream) | 82 bp | 55.00 | 60.25 ℃ |
| CTTCCACCTTGGAGCACTGT (Downstream) | 55.00 | 59.89 ℃ | ||
| P38 | GGCAGGAGCTGAACAAGACA (Upstream) | 92 bp | 55.00 | 60.25 ℃ |
| AGCACACACAGAGCCATAGG (Downstream) | 55.00 | 59.75 ℃ | ||
| CSF2 | GAAACTTCCTGTGCAACCC (Upstream) | 121 bp | 52.63 | 56.73 ℃ |
| CATCTGGCCGGTCTCACTC (Downstream) | 63.16 | 59.86 ℃ | ||
| AP-1 | TCGCTGCCTCCAAGTGCCGAAA (Upstream) | 134 bp | 59.09 | 67.20 ℃ |
| AAGCTGTGCCACCTGTTCCCT (Downstream) | 57.14 | 64.32 ℃ | ||
| CREB5 | AAGACTGCCCAATAACAGCC (Upstream) | 150 bp | 50.00 | 58.16 ℃ |
| CCACCTCGCTGACCGATG (Downstream) | 66.67 | 60.20 ℃ |