FIG. 1.

Coimmunofluorescence of Ste6p and Kex2p. HA-tagged Ste6p and Kex2p were expressed from the GAL1 promoter from the centromeric-based plasmids pSM1361 and pBMKX22 (provided by R. Fuller), respectively, in strain SM2544 (MATa ste6-Δ4). Cells were processed for immunofluorescence after induction in galactose as described in Materials and Methods. Ste6p was detected with the mouse anti-HA antibody 12CA5, and Kex2p was detected with the rabbit anti-Kex2p antibody. Rhodamine-conjugated anti-mouse and FITC-conjugated anti-rabbit secondary antibodies were used to visualize the Ste6p and Kex2p staining patterns, respectively.