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. 2024 Jan 26;30(2):382–393. doi: 10.1038/s41591-023-02788-5

Fig. 2. Novel antibody shows specificity for HDGFL2 with cryptic peptide.

Fig. 2

a, TDP-43 is reduced (arrowhead) in HeLa treated with TDP-43 siRNA (siTDP) compared with nontransfected control HeLa (ctrl). Ab, antibody. b, Reverse transcription PCR using primers designed to amplify the cryptic exon sequence of HDGFL2 shows a product (arrowhead) found only in siTDP. c, Protein extracts as in a were subjected to protein blot analysis using either an antibody (rabbit polyclonal antibody against human CTB-50L17.10, HPA044208) against the native HDGFL2 protein (left) or the novel monoclonal antibody (TC1HDG) against the cryptic sequence in HDGFL2 (right). While WT HDGFL2 was detected in both control and siTDP lysates (left and right bands at level of left arrowhead), HDGFL2 harboring the neoepitope (right arrowhead) was detected only in siTDP. d, IP blot using TC1HDG cryptic antibody for pulldown and WT HDGFL2 antibody for blotting revealed a band of the expected size only in siTDP (arrowhead). Lower bands represent presumable IgG heavy and light chains.