Fig. 3. TC1HDG antibody detects cryptic HDGFL2 in neurons of the ALS–FTD brain.

Cryptic HDGFL2 was detected by our TC1HDG cryptic antibody (first row, yellow) specifically in neurons (arrowheads) of the motor cortex (first column) or hippocampus (third column) that are depleted of nuclear TDP-43 (second row, green) and contain phosphorylated TDP-43 (pTDP-43) cytoplasmic aggregates (third row, red). Note that cryptic HDGFL2 immunoreactivity is largely restricted to the nuclear compartments. Neurons with intact nuclear TDP-43 did not show cryptic HDGFL2 immunoreactivity. Scale bars, 20 µm.