Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 24;8(2):165–176. doi: 10.1038/s41551-023-01065-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a, Normalized allele frequency changes in categories (in-frame indels, synonymous, non-sense and frame-shift mutations) in exon 11 assayed in diploid and BRCA2 loHAP hiPSCs, represent as log2 (day 21/day 7). P values report the significant comparisons between diploid and BRCA2 loHAPs in each category, in two-tailed Student’s t-test. Centre showing mean and error bars showing SEM. n represents biological replicates. b, Quantification of categorized allele frequencies (unedited, in-frame indels, HDR alanine substitution and frame-shift mutations) in exon 2 assayed in diploid and BRCA2 loHAP hiPSCs and hESCs at day 7 and 14 after editing. Bars showing mean of 2 or 3 biological replicates and error bars showing SEM. c, Allele frequencies of designer mutations in BRCA2 exon 2 alanine scan in diploid hPSCs at day 7. Bars showing mean of 3 biological replicates and error bars showing SEM. d, Same as in c, for BRCA2 loHAP hPSCs. e, Quantification of the relative depletion of in-frame and frame-shift mutations in different frequency groups in BRCA2 loHAPs. Cells were edited in exon 2 performing alanine scanning as described in Fig. 3d–g and samples collected at day 7 and 21 after editing in BRCA2 loHAP hPSCs. Frame-shift and in-frame alleles were binned into three groups based on their allele frequency (>0.5%, 0.05–0.5% and <0.05%) and the bars showing median and whiskers showing interquartile range. f, Allele frequency changes overtime of mutations generated in BRCA2 exon 2 alanine scan in diploid hPSCs, represented as log2(day 14/day 7), circle and log2 (day 21/day 7), triangle. Bars showing mean and error bars showing SEM. Data points represent biological replicates. g, Normalized allele frequency changes upon treatment of PARP inhibitor, Niraparib, of BRCA2 exon 2 alanine mutations in diploid hPSCs, represented as log2(drug/mock). 1 µM, circle; 2 µM, triangle. Allele frequencies were normalized against corresponding WT. Bars showing mean and error bars showing SEM. Data points represent biological replicates. h, Same as in g, for Olaparib treatment.

Source data