Table 1. β-Galactosidase expression observed with the WT or mutant T7 RNAP and with the lac or g10 ITS.
RNAP | ITS | β-Galactosidase, activity* |
---|---|---|
WT | g10 | 19,000 |
I810S | g10 | 5,300 |
WT | lac | 3,400 |
I810S | lac | <15 |
I810S P266L | lac | 420 |
Given in nmoles ortho-nitrophenyl-β-d thiogalactopyranoside (ONPG) hydrolized per min per mg of protein.