Fig. 4.

Mapping of VAP domains involved in the interaction with MP. (A) VAP and VAP deletion mutants are depicted by boxes, with the construct names indicating the corresponding amino acid positions. The coiled-coil region is shaded black. (B) Lanes 1-3, interaction of GST-MP (295-327) with VAP and its mutants. Lane 4, VAP alone. Proteins were separated by SDS/PAGE and detected by Western blotting with antibodies against VAP. The position of the 14.3-kDa marker (lysozyme) is on the left of the gel.