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. 2005 Apr 18;102(17):6143–6147. doi: 10.1073/pnas.0408171102

Fig. 2.

Fig. 2.

Neurons from adult male mice containing an extra X chromosome were retrogradely labeled with FluoroGold. (A) Dorsal view schematic showing FluoroGold injections in the right olfactory bulb and left motor cortex. (B) Cross section from A showing location of back-filled aneuploid neurons (box 1 corresponds to cell in C and box 2 corresponds to the cell in F). (C and F) Z stacks from a deconvolution microscope showing hyperploid cells (white arrows) in the adult secondary motor cortex (C) and lateral orbital cortex (F) that contain an extra X (red) chromosome. Neighboring cells are euploid for the sex chromosomes. Nuclei are stained blue with DAPI. (D, E, G, and H) Cytoplasmic localization of FluoroGold label (black arrows) in the aneuploid neurons from C and F by using Nomarski (D and G) and brightfield (E and H) microscopy. (Scale bars, 10 μm.) AI, agranular insular cortex; AOM, anterior olfactory nucleus, medial; AOV, anterior olfactory nucleus, ventral; Cg1, cingulate cortex, area 1; DEn, dorsal endopiriform nucleus; IL, infralimbic cortex; LO, lateral orbital cortex; M1, primary motor cortex; M2, secondary motor cortex; MO, medial orbital cortex; Pir, piriform cortex; PrL, prelimbic cortex; VO, ventral orbital cortex.