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. 2024 Feb 20;15:1541. doi: 10.1038/s41467-024-45829-4

Fig. 3. The majority of low abundance Parkin variants are thermolabile proteasome targets.

Fig. 3

Representative histograms for landing pad cells expressing the PRKN library untreated (-) (n = 559,000 cells) or treated (+) (n = 436,000 cells) with (A) 15 μM bortezomib (BZ) for 16 hours, (B) 25 nM siRNA against PSMD14 (siPSMD14) (n = 426,000 cells) or 25 nM control siRNA (siCTRL) (n = 506,000 cells) for 48 hours, or (C) without (-CQ) (n = 495,000 cells) or 20 μM chloroquine ( + CQ) (n = 516,000 cells) for 16 hours. Zoom ins, defined by black squares, are shown for the BZ and siPSMD14 histograms. (DE) Flow cytometry profiles for landing pad cells expressing the PRKN library and grown at (D) 37 °C (n = 516,000 cells) or 39.5 °C (n = 511,000 cells) for 16 hours, or (E) 37 °C (n = 516,000 cells) or 29 °C (n = 511,000 cells) for 16 hours. F Representative flow cytometry profiles for landing pad cells expressing the PRKN library untreated (-) (n = 407,000 cells) or treated (+) (n = 426,000 cells) with 10 μM Parkin activator BIO-2007817 for 24 hours.