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Complementarity between Ty1 boxes 0, 1, and 2.1 and tRNAiMet. Mutations (nucleotides in white on black) in Ty1 RNA or tRNAiMet that disrupt base pairing between the boxes and the complementary sequences of tRNAiMet impair transposition. The transposition defect is overcome when base pairing between mutant (mut) tRNAiMet and mutant Ty1 RNA is restored by compensatory mutations. Transposition frequencies of the Ty1 RNA-tRNAiMet combinations shown here are listed in Table 1. wt, wild-type.
