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. 2005 Apr 13;102(17):6057–6062. doi: 10.1073/pnas.0408666102

Fig. 3.

Fig. 3.

Sequence analysis of ΔmtDNA purified from skeletal muscles of an F3 mito-mouse. (A) Gene maps of wild-type mtDNA of M. spretus (green circle) and ΔmtDNA of M. m. domesticus (smaller red circle). The open arc in the ΔmtDNA map corresponds to the deleted region expanded from tRNALys to ND5 genes (np 7,759-12,454). No MluI site is present in wild-type mtDNA of M. spretus, whereas ΔmtDNA of M. m. domesticus possesses one MluI site. (B) Sequences of the cloned ΔmtDNA purified from skeletal muscles of an F3 mito-mouse possessing 30.7% ΔmtDNA. Green and red lanes correspond to mtDNA sequences of M. spretus and M. m. domesticus (38), respectively. Of 210 clones of ΔmtDNA from skeletal muscles, two clones (clones 17 and 18) possess two regions carrying sequences specific to M. spretus mtDNA in np 3,350-3,386 (ND1 gene) and np 4,093-4,213 (ND2 gene), and one clone (clone 19) possesses a region carrying sequences specific to M. spretus mtDNA in np 6,627-6,638 (CO1 gene). Of the remaining 207 clones, 191 possess completely identical sequences to those of M. m. domesticus mtDNA, whereas 16 clones (clones 1-16) possess somatic mutations. (C) Gene maps of clones 17 and 18 (Left) and clone 19 (Right). Green and red regions correspond to sequences of M. spretus and M. m. domesticus mtDNAs, respectively.