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. 2024 Feb 20;7:209. doi: 10.1038/s42003-024-05890-7

Fig. 2. BECLIN1 deletion leads to loss of differentiated intestinal epithelial cell subpopulations.

Fig. 2

a The proliferative capacity of cells in the intestinal stem cell compartment and transit-amplifying regions (black arrows), remains intact when BECLIN1 or ATG7 is absent. FFPE sections of small intestines were stained with specific markers for the various IEC subpopulations. BECLIN1 is critical for the maintenance of specific IEC subpopulations such as b goblet (Periodic acid-Schiff-Alcian Blue, PAS/AB staining) and c tuft (doublecortin like kinase 1, DCLK1, staining) cells, but not others such as d enteroendocrine (chromogranin A, ChgA staining) and e Paneth (lysozyme, Lyz staining) cells. Quantitation of these specific IECs is represented in graphs on the right. All data represent at least n = 4 biologically independent mice per genotype from n = 3 independent experiments. Graph shows the mean ± S.E.M. Significance determined by Welch’s unpaired t-test. ns = not significant (p > 0.05). EEC: enteroendocrine cells.