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E804 blocks tyrosyl phosphorylation of Src and Jak1 in cells. MDA-MB-468 cells were treated with 10 μM E804 in a time-dependent manner (A and C) or with E804 for 4 h in a dose-response manner (B and D). DMSO was used as vehicle control. PD180970 (1 μM) was used for comparison (D, lane 5). Seventy micrograms of cell-free extracts was subjected to immunoblotting with specific antibodies to p-Src, c-Src, p-Jak1, or Jak1. Positive immunoreactivity was detected by using ECL.
