Fig. 5. P1-like MGEs have 17 gene families in common.

A The screen for homologous P1-like elements resulted in 45 plasmids and 12 integrative prophage regions, of which some are closely and others are weakly related. B Closely related elements (having at least 75% of conserved gene families of the P1 subgroup1) were grouped (119 P1g1 P-Ps, 38 plasmids and 2 integrative prophage regions) and 17 persistent genes (present in more than 90% of the elements) were identified using PanACoTA (see “Methods”). C Pangenome graph of the P1 subgroup1 (P1g1)³². The conserved gene families of the P1-like elements (from B) are labeled as dark blue nodes. Gray nodes correspond to other conserved gene families (large nodes with high and small with intermediate frequency in all genomes) in the pangenome of P1g1. Edges represent the conservation of the genetic neighborhood. If an edge is assigned between two nodes, then the respective genes are co-localized.