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. 2005 Apr 12;102(17):6160–6165. doi: 10.1073/pnas.0500468102

Fig. 3.

Fig. 3.

Cellular distribution and FRET after expression of fluorophoretagged Kv2.1 and Kv9.3 subunits. (A) Confocal fluorescence images of a representative cell coexpressing YFPKv2.1 (Upper) and CFPKv2.1 (Lower) before (pre) and after (post) photobleaching of the acceptor in the indicated region. (B) Relative fluorescence intensity measured from images taken at different times during the photobleaching of the perimeter of the cell within the region of interest indicated in A. (C and D) Data acquisition and analysis are analogous to A and B for a representative cell-expressing YFPKv9.3 and CFPKv9.3. The images denoted pre and post in A and C correspond to image numbers 2 and 5 in the plots, respectively. (E) Scatter plots of the increase of CFP and decrease of YFP fluorescence intensity during acceptor photobleaching from B (○) and D (•). (F) ID for complete bleaching of the acceptor was obtained by regression and used to calculate the FRET efficiency (E) for cells coexpressing YFPKv2.1 and CFPKv2.1 (n = 16) or YFPKv9.3 and CFPKv9.3 (n = 11).