Figure 5.

Schematic idiograms of more distant species when compared to Rhynchospora breviuscula, showing FISH signals from the Rbv-I (green) and Rbv-II (magenta) oligo-probes. (A) Only a few signals were observed in R. ridleyi, hampering ortholog identifications. In R. cephalotes (B) and R. alba (C), despite the lack of correlation to the R. breviuscula barcode pattern, the low number of signals observed suggest that the high chromosome number might have derived from chromosome fissions, rather than polyploidy. In both species of clade V, R. holoschoenoides (D) and R. barbata (E) the signals were found dispersed, likely due to lack of specificity, despite sharing the same chromosome number as R. breviuscula (n = 5).