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[3H]CPO (1 nM) labeling of mouse brain membranes (200 μg of protein) in vitro showing the proposed diethylphosphorylated (a), aged (b), and hydrolyzed (c) portions. Time course is shown as dpm (A) and percent of total dpm added (B). In the aged curve (b), potassium fluoride was added for 15 min after the incubation times indicated. Data are mean ± SD (n = 3).
