Skip to main content
. 2024 Feb 20;7:208. doi: 10.1038/s42003-024-05823-4

Fig. 2. Praja2 is required for receptor endocytosis.

Fig. 2

a HeLa cells were transfected with siRNAs (control or targeting praja2), serum-deprived overnight, and treated with EGF (10 ng/µl) for 90 min. Cells were PFA-fixed and immunostained with anti-EGFR antibody (green) and DAPI (blue). Representative confocal images are shown. Scale bar is indicated. b Quantitative analysis of experiment shown in (b). A mean value of three independent experiments ± SD is shown. c Same as in (a), with the exception that cells were lysed and immunoblotted for EGFR, praja2, and α-tubulin. d Quantitative analysis of experiment shown in (b). A mean value of three independent experiments ± SD is shown. Student’s t-test *P = 0.02 for 60 min, *P = 0.014 for 90 min time point. e HeLa cells were transfected with siRNAs (control or targeting praja2), serum-deprived overnight, and treated with transferrin (Tf, 2 µg/ml) for 60 min. Cells were PFA-fixed and immunostained with anti-TfR antibody (green) and DAPI (blue). Representative confocal images are shown. Scale bar is indicated. f Quantitative analysis of experiment shown in (b). A mean value of three independent experiments ± SD is shown. g HeLa cells were transfected with siRNAs (control or targeting praja2), serum-deprived overnight, and treated with EGF (10 ng/µl) for 5 min. Cells were PFA-fixed and immunostained with anti-AP2m antibody (green) and DAPI (blue). Representative confocal images are shown. Scale bar is indicated.