Fig. 1.

Palmitoylation of the Kv1.1. ion channel. Sf9 cells infected with recombinant Kv1.1-containing virus or control virus were incubated in the presence of 2 mCi of [³H]palmitic or [³H]myristic acid for 2 h at 22°C. Immunoblot analysis of immunoprecipitated solubilized whole cell lysates by using rabbit polyclonal antisera demonstrated equal amounts of immunoreactive Kv1.1 in Kv1.1-infected Sf9 cells (lanes 2 and 3) whereas the control virus-infected cells (lane 1) did not contain any immunoreactive material. Autoradiographs of immunoprecipitated, radiolabeled samples detected a radioactive doublet only in the Kv1.1-infected, palmitate-labeled sample (lane 3) but not in either the control virus-infected, [³H]palmitate-labeled sample (lane 1) or the Kv1.1-infected, [³H]myristate-labeled sample (lane 2). These data are representative of the results of three independent experiments.