Fig. 3.

Macroscopic current kinetics of the C243A Kv1.1 channel mutant. (A and B) Whole-cell, voltage clamp recordings with depolarizing voltage steps from -40 to +40 mV (10-mV increments) from a holding potential of -80 mV were performed on infected Sf9 cells expressing WT-Kv1.1 (A) or C243A mutant protein (B). (C) Comparisons of WT-Kv1.1 (□) and C243A (▪) current-voltage relationships. Notably, C243A currents demonstrated a leftward shift of 20 mV in the current-voltage relationship in comparison to WT protein. Error bars represent the x̄ ± SE of three independent preparations.