FIG. 8.

Determination of the region of AML1-MTG8 which is required for interaction with MTGR1. L-G cells were infected with LXSH-Flag-MTGR1 and LNSX-retrovirus, which encodes either wild-type or mutant HA-tagged AML1-MTG8. Control cells (vector) expressed only the Flag-tagged MTGR1. Immunoprecipitations were performed with anti-HA monoclonal antibody (C and D) or anti-Flag monoclonal antibody (B). The immunoprecipitates and total-cell lysates (A) were separated on SDS–10% polyacrylamide gels and analyzed by immunoblotting with anti-MTGR1 polyclonal antibody (D) or anti-MTG8 polyclonal antibody (A to C).