Fig. 1.

Three-dimensional screening. A matrix of data was obtained by altering cell states, small-molecule perturbagens, and cell measurements. Four cell lines, which model tumorigenic conversion and were generated by Weinberg and coworkers (BJ fibroblast cells serially transduced with the four indicated oncogenes), were treated with five small-molecule perturbagens (wortmannin, rapamycin, 2-deoxyglucose, oxamic acid, and oligomycin). Biochemical signatures of the members of this matrix were calculated by using metabolic measurements such as oxygen consumption, glucose consumption, lactate production, and metabolite measurements, using GC-MS or HPLC.