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Alignment of the putative Gpa2-binding domain from Git2 with sequences of other fungal adenylate cyclases. Plus and minus signs indicate the positions of nine conserved residues in Git2 subjected to alanine substitution and the resulting effect on the ability to interact (+) or not (-) with the Gpa2R176H bait in the two-hybrid assay. The residue numbers follow each entry used in the alignment along with the size of the adenylate cyclase protein in parentheses.
