Table 3.
Prenatal MeHg exposure and DNA methylation. Meta-analysis of epigenome-wide association study including highlighted CpG sites with a Pmeta<10−5.
| Model | CpG site | Beta | SE | p-value | I2 | Studies | Sample | Effects | FDRa | Chr. | Position | UCSC RefGene Name | Location |
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| Child blood DNAm - Without fish | cg02212000 | −0.0008 | 0.0002 | 8.13E-07 | 0 | 4 | 794 | - | 0.1011 | chr8 | 63951669 | GGH | TSS200 |
| Child blood DNAm - With fish | g02212000 | −0.0008 | 0.0002 | 3.65E-06 | 0 | 4 | 739 | - | 0.4540 | chr8 | 63951669 | GGH | TSS200 |
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| Cord blood DNAm - With fish | cg24184221 | 0.0002 | 0.0001 | 5.87E-05 | 0 | 5 | 1462 | +-+++ | 1.0000 | chr17 | 6,555,443 | MED31; C17orf100 | Exon |
| Child blood DNAm - With fish | cg15288800 | 0.0036 | 0.0009 | 4.97E-05 | 0.3014 | 4 | 739 | ++++ | 0.5133 | chr17 | 6,555,742 | MED31; C17orf100 | 3’ UTR |
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| Child blood DNAm - Without fish | cg12204245 | 0.0020 | 0.0004 | 4.81E-07 | 0.6585 | 4 | 794 | ++++ | 0.0898 | chr13 | 114,321,214 | GRK1 | TSS1500 |
| Child blood DNAm - With fish | cg12204245 | 0.0022 | 0.0004 | 1.31E-07 | 0.4678 | 4 | 739 | ++++ | 0.0489 | chr13 | 114,321,214 | GRK1 | TSS1500 |
Shown are the 4 highlighted CpGs ordered by p-value. Results presented per 1 μg/L increase in prenatal MeHg exposure (cord blood total Hg concentration or child total Hg concentration). Column heads: Beta: regression coefficient; SE: standard error for regression coefficient; p-value: unadjusted p-value for the regression coefficient; I2: heterogeneity statistics; Studies: number of studies involved; Sample: sample size in the model; Effects: direction of effect across cohorts included in the statistical model (ALSPAC, INMA, MoBa, and RHEA): Hg exposure during pregnancy was associated with increased (+) or decreased (−) methylation, or missing (?) result; FDR: false discovery rate; Chr: chromosome; Position: chromosomal position based on NCBI human reference genome; UCSC RefGene Name: UCSC annotated gene.
All models were adjusted for maternal age, parity, education, smoking during pregnancy, and child sex, and cellular heterogeneity.
Mapped from the array to the hg19 genome provided by Illumina. Location of differentially methylated sites has been added according to the IlluminaHumanMethylation450kanno.ilmn12.hg19 R package.
TSS: Transcription Start Site; UTR: untranslated region.
Epigenome-wide significance threshold (FDR p ≤ 0.1).