Figure 1.

Gastric Muscle Immune Cell Atlas from Healthy Volunteers and Patients with Idiopathic Gastroparesis

(A) Schematic representing the dissociation and isolation of CD45⁺ cells from mucosa deprived muscle layer for scRNA sequencing.

(B) Immunohistochemistry showing the distribution of CD45⁺ cells (arrowheads) and CD31 (PECAM1) immunoreactivity (arrows). CD45⁺ cells were noted both in proximity to and away from CD31 immunoreactivity. Also shown is colabeling with PGP9.5, a marker for enteric neurons and nerve fibers. Scale bar = 50 μm.

(C) T-sne graph of all called cell types in the cohort, 13 controls and 7 patients with gastroparesis. Cell types called represented as T cells, myeloid, and B cell compartments. T-sne graph of T cells colored by CD4⁺ T cells (red), CD8⁺ T cells (green), natural killers (NKs, purple), and ILCs (blue). T-sne graph of myeloid cells colored by macrophages (green), mast cells (blue), monocytes (purple), and dendritic cells (DC, red). T-sne graph of B cells color by B cells (red), follicular (blue), and cycling B cells (green).

(D) Percentage of patient contribution to each cell type colored by disease status (control patients are blue, dark blue, and purple; patients with gastroparesis are red, orange, and yellow). Color shades in the respective bars are to represent contribution of unique patient samples.

See also Figures S1–S3 and Tables S1, S2, and S3.