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. 2024 Jan 20;23(2):100723. doi: 10.1016/j.mcpro.2024.100723

Fig. 4.

Fig. 4

Knockdown of Anxa2 affected the inflammatory response genes and pathways upon OGD/R in BV2 microglial cells.A, schematic of Anxa2 encoding region and target site of a stable cell line of Anxa2 knockdown by small hairpin RNA (shRNA) in BV2 cells. B, RT-PCR analysis for Anxa2 mRNA expression in BV2 stable cell lines of small hairpin RNA control (shCtrl) and small hairpin RNA of Anxa2 gene (shAnxa2), respectively. C, representative blot images and quantitative analysis of exogenous Anxa2 protein in shCtrl and shAnxa2 BV2 stable cell lines detected by Western blot. Data are presented as means ± SEM. Statistical significance was determined by unpaired Student’s t-tests. ∗∗∗∗p < 0.0001. D, differential gene expression volcano plots and (E) Venn diagram revealed significant changes in BV2 microglial cells transcriptome of Anxa2 knockdown after OGD/R (p-value <0.05, fold change >1.5). F, biological process analyses of DEGs upregulated in shCtrl-group after OGD/R (p-value <0.05). G, the biological process of DEGs downregulated in shAnxa2-OGD/R versus shCtrl-OGD/R group (p-value <0.05). Anxa2, annexin A2; DEG, differentially expressed gene; OGD/R, oxygen-glucose deprivation and reoxygenation.