Fig. 7.

Anxa2 knockdown alleviates OGD/R-induced neuronal injury.A, schematic diagram of primary neurons treated by BV2 microglia cells-conditional medium (CM) in vitro for the following assays. B, cell viability of primary neurons treated with conditional medium from shCtrl or shAnxa2 BV2 cells with or without OGD/R treatment using MTT assay. C–E, representative blot images (C) and quantitative analyses of cleaved caspase3 (D) and Bax (E) in neurons treated with conditional medium derived from shCtrl or shAnxa2 BV2 cells with or without OGD/R treatment. GAPDH was an internal normalized control. F and G, representative images (F) and quantitative analyses (G) of TUNEL (green) and NeuN (red) positive neurons subjected to OGD with conditional medium derived from shCtrl or shAnxa2 BV2 cells with or without OGD/R treatment. The arrow indicates the colocalization of TUNEL and NeuN. Scale bar represents 10 μm. n = 10. All data are shown as mean ± SEM. Statistical significance was determined by two-way ANOVA for multiple comparisons, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. All experiments were repeated at least three times. Anxa2, annexin A2; MTT, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; OGD/R, oxygen-glucose deprivation and reoxygenation.