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. 2024 Feb 6;27(3):109152. doi: 10.1016/j.isci.2024.109152

Figure 3.

Figure 3

PCID2 blocks post-transcriptional steps of HIV-1 latency

(A) Schematic figure of the HIV-1 genome in J-Lat 11.1. Arrows represent primer location and amplicons across the HIV-1 genome used in the transcriptional profiling assay.

(B) Transcriptional profiling assay of shControl and shPCID2 J-Lat 11.1. Gene expression blocks at post-transcriptional steps are assessed by calculating the ratio of the relative abundance of HIV-1 RNA species as shown figure and A. Data are presented as fold change in HIV-1 RNA ratios in shPCID2 relative to shControl. Bars represent mean of 5 independent shRNA-mediated knockdown experiments and error lines represent SEM. Statistical significance was determined by t test; ∗p < 0.01.

(C) Representative FISH-Flow plots of shControl, shPCID2, or shIKZF1 knockdown J-Lat 11.1 cells.

(D) Bar graph showing fold change in vRNA+GFP-, vRNA-GFP+, and vRNA+GFP+ percentages in shControl, shPCID2, and shIKZF1 cells. Bars and error lines represent mean and SEM (n = 3). Statistical significance was determined by t test, ∗p < 0.05.

(E) Percentage of GFP+ cells that express viral RNA (vRNA+) in shControl, shPCID2, and shIKZF1 as analyzed by FISH-Flow. Bars and error lines represent mean and SEM respectively. Statistical significance was calculated by t test; ∗p < 0.05.