Table 1.
List of primers used for quantitative PCR
| Amplicon | Forward primer 5′-3′ | Reverse primer 5′-3′ |
|---|---|---|
| Nuc 0 | CCACACACAAGGCTACTTCC | AACTGGTACTAGCTTGTAGCAC |
| HSS1 | TGTGAGCCTGCATGGGATGG | GAAAGTCCCCAGCGGAAAGT |
| Nuc1-HSS2 | CGTCTGTTGTGTGACTCTGGT | TCGAGAGAGCTCCTCTGGTT |
| HSS2 | GCCCGAACAGGGACTTGAAA | TTGGCGTACTCACCAGTCG |
| Nuc2 | GGTGCGAGAGCGTCAGTAT | AGCTCCCTGCTTGCCCATA |
| HK2 promoter | GCCGACTCTTGTATTGCCTG | TATTGTAGCACGGCCGGAAA |
| PCID2 | AACACACCAGCTGTCTCTGG | GCCTTTGACGTGTCCCATGT |
| GFP | GAAGCAGCACGACTTCTTCAA | GCTTGTCGGCCATGATATAGA |
| Readthrough | GCCCTCAGATGCTGCATATAA | AGAGTCACACAACAGACGG |
| TAR | GTCTCTCTGGTTAGACCAG | TGGGTTCCCTAGTTAGCC |
| UTR | GCTTCAAGTAGTGTGTGCCC | GAGAGCTCCTCTGGTTTCCC |
| Tat-Rev/CS vRNA | GACTCATCAAGTTTCTCTATCAAA | AGTCTCTCAAGCGGTGGT |
| US vRNA | GACGCTCTCGCACCCATCTC | CTGAAGCGCGCACGGCAA |
| SS vRNA | GCGGCGACTGGAAGAAGC | CTATGATTACTATGGACCACAC |
| 18S RNA | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
| B2-microglobulin | AGCGTACTCCAAAGATTCAGGTT | ATGATGCTGCTTACATGTCTCGAT |
| GAPDH | GCAGGGGGGAGCCAAAAGGG | TGCCAGCCCCAGCGTCAAAG |
| PPIA | TCATCTGCACTGCCAAGACTG | CATGCCTTCTTTCACTTTGCC |